Buy Online
Buy Online

Products

Culture Media Ingredients

bacteriologycard image

Overview

High-standard bacteriological grade Agar Agar powders. Perfect as a Culture Media

For specific use-cases, the culture medium for bacterial growth can be either liquid, solid or semi-solid. Agar-agar is a popular choice as culture media as it is a good way to form solid or semisolid medium.

Culture Media gives artificial environment simulating natural conditions necessary for the growth of bacteria. The basic requirements for Culture Media are :

  • Energy/Carbon/Nitrogen Source
  • Salts
  • Optimum pH
  • Adequate Oxydation-reduction potential

Packing

Bulk : 5, 10, 15, 20, 25 kg cartons & 25kg fibreboard drum

packcard image

Applications

    BIOTECHNOLOGY

    Agar Agar

    Agar Agar is an indispensable part of the biotechnology industry. It is used as a gelling agent to prepare semi-solid and solid plant tissue culture medium. The reason why Agar is preferred over other gelling agents are:

     Workable at incubation temperature

    Agar Agar when mixed with appropriate portions of water forms into a gel which melts at around 85-95° C and solidifies at 34°C to 41°C.

     Nonreactive with media constituents

    Agar-Agar remains unfaced when subjected to media constituents and is immune from plant enzymes.

     Controllable firmness

    The firmness of Agar gel depends on the concentration, pH of the medium, and the Agar brand used as culture medium. The Agar concentrations that generally range between 0.5% to 1.0% give the required firmness at a pH specific for plant cell culture media.

    Agar gels are transparent, allowing for easy observation of cells or microorganisms during growth and experiments.

    Agar is also used in plant tissue culture to provide a solid medium for the growth and regeneration of plant cells, tissues, and organs. It is frequently employed for techniques like micro propagation and somatic embryogenesis.

    Researchers can adjust the concentration of agar to control the gel’s firmness and the density of the culture medium. This flexibility allows them to create custom media tailored to the specific requirements of their tissue culture experiments.

    Agarose

    Agarose, an Agar Agar derivative is also extensively used for various biotechnological applications:

    • For Gel chromatography as a separator of large molecules like antibodies, lipo proteins,etc.
    • For DNA and RNA recoveries.
    • For live cell imaging, as it improves the quality of images.

    Plant Gel (Gellan Gum)

    Gellan Gum is used for suspension and plant tissue culture applications.

    Gellan gum is used to create a gel-like medium that provides mechanical support to plant or microbial cells. This is particularly useful in tissue culture when you need a solid or semi-solid medium for the growth of cells.

    Gellan gum allows for the control of the consistency of the culture medium. By adjusting the concentration of Gellan gum, you can achieve different levels of gel strength and viscosity, which can be tailored to the specific requirements of your tissue culture.

    The gel created by Gellan gum retains moisture and nutrients, making it an ideal medium for cell growth. It ensures that cells have access to the necessary nutrients while preventing them from settling at the bottom of the container. gellan gum-based gels are generally transparent, which allows for easy observation and monitoring of cell growth and development under a microscope.

     

    BIO FERTILIZER

    For fruitful organic farming

    Biofertilizers are widely used in agriculture and revolve around creating microbes that eventually help the plants and trees grow better. The biofertilizer manufacturing process is highly dependent on microbes. Therefore, the quality and quantity of microbes is important.

    Agar can be an innovative alternative to synthetic polymers used in horticulture as it contains active bio stimulant compounds and also helps to hold water in the soil that aids plant growth with minimum water consumption.

    Therefore, Agar Agar and Agar derived products play a crucial role in organic farming.

    CULTURE MEDIA

    Agar Agar, the perfect culture media to grow all pathogenic and non pathogenic bacteria and fungi.

    Agar Agar

    Agar-Agar is known for its exceptional qualities as culture media in the Microbiology industry. It does not require the presence of any bacteria or fungi for its production. Therefore, it never inhibits the growth of these microorganisms, making it the best choice as culture media. Other prominent reasons for the same are:

    • Dissolving and cooling properties

    Agar-agar is derived from the cell walls of red seaweed. When dissolved in hot water the agar-agar powder transforms into jelly when cooled. The jelly structure makes it suitable as a culture medium when organisms need to be cultured in a petri-dish.

    • Higher melting point than gelatin

    Agar-agar does not melt at the temperature required for the growth of microorganisms(typically around 37°C). Therefore, it is easily possible to heat the petri-dish which is used to grow microorganisms, as the culture medium remains intact and catalyzes the process.